Thymoquinone Suppresses Cell Proliferation and Enhances Apoptosis of HL60 Leukemia Cells through Re-Expression of JAK/STAT Negative Regulators

Objective: The natural compound, thymoquinone (TQ) has demonstrated potential anticancer properties in inhibiting cell proliferation and promoting apoptosis myeloid leukemia cells, breast cancer others. However, the effect mechanism of TQ on AML cells still not fully understood. In this study, authors examined effects expression JAK/STAT-negative regulator genes SOCS-1, SOCS-3, SHP-1, their consequences HL60 cells. Methods: MTT trypan blue exclusion tests were conducted to determine 50% inhibitory concentration (IC50) proliferation. FITC Annexin Guava® reagent used study examine cycle phases, respectively. genes, was investigated using reverse transcriptase- quantitative PCR (RT-qPCR). Results: a inhibition significant increase apoptotic dose time-dependent manner. significantly induced arrest at G0-G1 phase (P < 0.001) enhanced re-expression genes. Conclusion: potentially inhibited increased with suggesting that could be new therapeutic candidate for therapy.